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Merck & Co bugbuster master mix
Bugbuster Master Mix, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bugbuster+master+mix/bugbuster+master+mix/pmc13069688-76-9-13
Average 86 stars, based on 1 article reviews
bugbuster master mix - by Bioz Stars, 2026-10
86/100 stars

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Centrifugation:

Article Title: High-affinity TCR for recognizing AFP antigen
Article Snippet: .. The cell pellets were harvested by centrifugation at 5000 rpm for 15 min, and the cell pellets were lysed with Bugbuster Master Mix (Merck). .. The inclusion bodies were recovered by centrifugation at 6000 rpm for 15 min, followed by washing with Bugbuster (Merck) to remove cell debris and membrane fraction.

Article Title: High-affinity TCR for AFP recognition
Article Snippet: .. The cell pellets were harvested by centrifugation at 5000 rpm for 15 mins, and the cell pellets were lysed with Bugbuster Master Mix (Merck). .. The inclusion bodies were recovered by centrifugation at 6000 rpm for 15 min, followed by washing with Bugbuster (Merck) to remove cell debris and membrane fraction.

Article Title: High-affinity TCR for AFP recognition
Article Snippet: .. The cells were washed once in PBS buffer and were dispensed, and cells corresponding to 200 mL of the bacterial culture were taken and lysed with 5 mL of BugBuster Master Mix (Merck), inclusion bodies were collected by centrifugation at 6000 g for 15 min and then washed with detergent for 4 times to remove cell debris and membrane components. ..

Article Title: High-affinity TCR for recognizing AFP antigen
Article Snippet: .. The cells were washed once in PBS buffer and were dispensed, and cells corresponding to 200 mL of the bacterial culture were taken and lysed with 5 mL of BugBuster Master Mix (Merck), inclusion bodies were collected by centrifugation at 6000 g for 15 min and then washed with detergent for 4 times to remove cell debris and membrane components. ..

Protease Inhibitor:

Article Title: LacI strikes a balance between stability and inducibility
Article Snippet: .. The pellet cells were then resuspended in 25 ml BugBuster ® Master Mix (Merck) solution containing one ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor pill (Roche), and the cell suspension was incubated on a shaking platform at 50–75 rpm for 10–20 min at room temperature. ..

Suspension:

Article Title: LacI strikes a balance between stability and inducibility
Article Snippet: .. The pellet cells were then resuspended in 25 ml BugBuster ® Master Mix (Merck) solution containing one ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor pill (Roche), and the cell suspension was incubated on a shaking platform at 50–75 rpm for 10–20 min at room temperature. ..

Incubation:

Article Title: LacI strikes a balance between stability and inducibility
Article Snippet: .. The pellet cells were then resuspended in 25 ml BugBuster ® Master Mix (Merck) solution containing one ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor pill (Roche), and the cell suspension was incubated on a shaking platform at 50–75 rpm for 10–20 min at room temperature. ..

Article Title: Genetic Code Expansion-Driven in Situ Click Labeling Enables Rapid Imaging-Based Selection of Functional Nanobody-Dye Conjugates.
Article Snippet: .. Once the OD reached 0.5, 0.2 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) (final) was added, and the culture was incubated at 18°C for 24 h. The culture was centrifuged, and the cell pellet was lysed with BugBuster Master Mix (Merck, Burlington, VT, U.S.A.) supplemented with 10 mM dithiothreitol (DTT). .. The lysate was cleared by centrifuging at 15000 × g for 15 min at 4°C and purified using His SpinTrap (Cytiva) according to the manufacturer’s instructions.

Membrane:

Article Title: High-affinity TCR for AFP recognition
Article Snippet: .. The cells were washed once in PBS buffer and were dispensed, and cells corresponding to 200 mL of the bacterial culture were taken and lysed with 5 mL of BugBuster Master Mix (Merck), inclusion bodies were collected by centrifugation at 6000 g for 15 min and then washed with detergent for 4 times to remove cell debris and membrane components. ..

Article Title: High-affinity TCR for recognizing AFP antigen
Article Snippet: .. The cells were washed once in PBS buffer and were dispensed, and cells corresponding to 200 mL of the bacterial culture were taken and lysed with 5 mL of BugBuster Master Mix (Merck), inclusion bodies were collected by centrifugation at 6000 g for 15 min and then washed with detergent for 4 times to remove cell debris and membrane components. ..



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